Intracellular Imaging, Inc.

Intracellular Imaging specializes in supplying technically sophisticated, easy to use, dependable, and cost effective, epi-fluorescence microscopy systems for live cell signaling studies. Our clients perform all types of dynamic live cell signaling analysis. But, far and away, the most popular use for our systems is for ratio-metric studies of intracellular calcium dynamics using FURA 2. It’s pretty simple why we recommend and supply DVC cameras for many of our systems. High performance at a very competitive price is the hallmark of Intracellular Imaging Inc.; and, DVC cameras are a big part of our ability to deliver on that.

The 340 monochrome is a particular favorite. I recommend it exclusively for our High Speed ratio-metric imaging systems. Our clients that do fast ratio-metric calcium analysis require outstanding speed and sensitivity. I don’t know of another camera that provides the speed and sensitivity of the DVC-340 monochrome at its price point. Combine that performance with the quality and service of DVC, and I consider the 340M an exceptional value.

Dave Will
President, Intracellular Imaging Inc.

These 3 images are Fibroblasts loaded with FURA 2 for determining dynamic calcium change. Fura 2 excites at 340nm light (left image) and 380nm light (center image). So we capture the image at 340 nm and the image at 380 nm. The ratio of the fluorescence intensity of 340/380 is the indicator of calcium concentration. In our software we have the ability to use a psuedocolor function and show the spatial resolution of the calcium. That is the third image (right). Low calcium is blue and high calcium is red.